Next Generation Sequencing Facilitates Quantitative Analysis of mRNA expression of STAT3 deficiency skin

Dominant-negative mutations of signal transducer and activator of transcription 3 (STAT3) signaling which controls epithelial proliferation in various tissues, leads to atopic dermatitis (AD) in hyper IgE syndrome (HIES). We found that the STAT3 signaling in keratinocytes is required to maintain skin homeostasis through this transcriptome analysis. Overall design: Ear skin of skin specific-Stat3 sufficient or deficient mice were taken and analyzed the transcriptome using Illumina HiSeq1500.

调控多种组织上皮增殖的信号转导与转录激活因子3（STAT3）信号通路的显性负突变，可在高IgE综合征（HIES）中引发特应性皮炎（AD）。本研究通过本次转录组分析发现，角质形成细胞中的STAT3信号通路对于维持皮肤稳态不可或缺。整体实验设计：选取皮肤特异性Stat3基因充足或缺陷小鼠的耳部皮肤样本，采用Illumina HiSeq1500平台进行转录组测序分析。