Reducing end thiol-modified nanocellulose: Bottom-up enzymatic synthesis and use for templated assembly of silver nanoparticles into biocidal composite material

We provide here the underlying data of the publication "Reducing end thiol-modified nanocellulose: Bottom-up enzymatic synthesis and use for templated assembly of silver nanoparticles into biocidal composite material". Please find the abstract below.

Nanoparticle-polymer composites are important functional materials but structural control of their assembly is challenging. Owing to its crystalline internal structure and tunable nanoscale morphology, cellulose is promising polymer scaffold for templating such composite materials. Here, we show bottom-up synthesis of reducing end thiol-modified cellulose chains by iterative bi-enzymatic β-1,4-glycosylation of 1-thio-β-D-glucose (10 mM), to a degree of polymerization of ∼8 and in a yield of ∼41% on the donor substrate (α-D-glucose 1-phosphate, 100 mM). Synthetic cellulose oligomers self-assemble into highly ordered crystalline (cellulose allomorph II) material showing long (micrometers) and thin nanosheet-like morphologies, with thickness of 5–7 nm. Silver nanoparticles were attached selectively and well dispersed on the surface of the thiol-modified cellulose, in excellent yield (≥ 95%) and high loading efficiency (∼2.2 g silver/g thiol-cellulose). Examined against Escherichia coli and Staphylococcus aureus, surface-patterned nanoparticles show excellent biocidal activity. Bottom-up approach by chemical design to a functional cellulose nanocomposite is presented. Synthetic thiol-containing nanocellulose can expand the scope of top-down produced cellulose materials.

本数据集对应发表论文《还原端巯基修饰纳米纤维素：自下而上酶促合成及利用其模板组装银纳米颗粒制备抗菌复合材料》的原始数据，下文附该论文摘要。

纳米颗粒-聚合物复合材料是一类重要的功能材料，但其组装过程的结构调控颇具挑战。纤维素因其内部结晶结构与可调控的纳米级形貌，成为制备此类复合材料模板的极具潜力的聚合物支架材料。本研究通过以1-硫代-β-D-葡萄糖（10 mM）为底物，经双酶催化的迭代β-1,4-糖苷化反应，实现还原端巯基修饰纤维素链的自下而上合成：产物聚合度约为8，以供体底物α-D-葡萄糖-1-磷酸（100 mM）计算的产率约为41%。合成的纤维素寡聚体可自组装为高度有序的结晶相（纤维素Ⅱ型同质异形体）材料，呈现微米级长度、厚度5~7 nm的超薄纳米片形貌。银纳米颗粒可选择性附着并均匀分散于巯基修饰纤维素表面，负载产率≥95%，负载效率约为2.2 g银/g巯基纤维素。针对大肠杆菌（Escherichia coli）与金黄色葡萄球菌（Staphylococcus aureus）的测试表明，该表面负载纳米颗粒的复合材料展现出优异的抗菌活性。本研究报道了一种通过化学设计制备功能化纤维素纳米复合材料的自下而上路径。合成的含巯基纳米纤维素可拓展传统自上而下制备纤维素材料的应用范围。