Genome-wide location analysis of BMP (SMAD1) in mouse erythroid progenitors co-occupted with lineage specific regulators (GATA1, GATA2)

Lineage regulators direct BMP and Wnt pathways to cell-specific programs during differentiation and regeneration Mouse G1E or G1ER cells were cross-linked with formaldehyde for 20 min. DNA was enriched by chromatin immunoprecipitation (ChIP) and analyzed by Solexa sequencing. A sample of whole cell extract (WCE) was sequenced and used as the background to determine enrichment. ChIP was performed using an antibody against total Smad1 (Santa Cruz SC-7965), Gata1 (Santa Cruz SC-265), or Gata2 (Santa Cruz SC-9008). This represents the Mouse ChIP-seq portion of this dataset.

细胞谱系调控因子在细胞分化与再生过程中，将骨形态发生蛋白（BMP）与Wnt信号通路导向细胞特异性基因调控程序。本数据集的小鼠ChIP-seq部分以小鼠G1E或G1ER细胞为实验材料：将细胞经甲醛交联处理20分钟，通过染色质免疫沉淀（ChIP）富集DNA后采用Solexa测序进行分析；设置全细胞提取物（WCE）样本进行测序并作为背景以判定富集效果；实验使用的抗体分别为靶向总Smad1（Santa Cruz SC-7965）、Gata1（Santa Cruz SC-265）及Gata2（Santa Cruz SC-9008）的抗体。以上内容为本数据集的小鼠ChIP-seq实验部分。