The Evolving Immune Microenvironment of B-cell Acute Lymphoblastic Leukemia [human peripheral blood dataset]

Single-cell RNA-seq analysis of primary human peripheral blood mononuclear cells (PBMCs) from two independent B-ALL patients (Texas Children's Hospital) and healthy PBMC donor samples (STEMCELL Technologies). Overall design: Single-cell RNA-sequencing libraries of primary human peripheral blood immune populations. B-ALL patient bone marrow samples were separated into CD19+ B cell and CD19-CD45+ non-B cell fractions by flow-based sorting and then mixed at a ratio of 1:5 CD19+:CD19-CD45+ followed by scRNA-Seq. Total CD45+ peripheral blood cells were sequenced from healthy donors.

本数据集针对来自德克萨斯儿童医院（Texas Children's Hospital）的2名独立B系急性淋巴细胞白血病（B-cell Acute Lymphoblastic Leukemia, B-ALL）患者的原代人外周血单个核细胞（peripheral blood mononuclear cells, PBMCs），以及STEMCELL Technologies公司提供的健康人外周血单个核细胞供体样本，开展单细胞RNA测序分析。整体实验设计：针对原代人外周血免疫细胞群体构建单细胞RNA测序文库。B-ALL患者的骨髓样本经流式分选分为CD19阳性B细胞与CD19阴性CD45阳性非B细胞组分，随后以1:5的比例（CD19阳性:CD19阴性CD45阳性）混合，再进行单细胞RNA测序（scRNA-Seq）。健康供体的总CD45阳性外周血细胞均完成测序。