a8dcf04b-5e92-41ff-ba58-bb1d86997ac6 - samples

Dataset contains targeted sequencing data of 712 plasma cell free DNA samples and 428 white blood cell samples collected from 428 men with metastatic prostate cancer. Target capture was performed using a hydridization-based custom Roche SeqCap EZ Choice kit, designed to capture all exons of 72 prostate cancer driver genes. Cell free DNA was extracted from 10 mL blood samples. Libraries were sequenced using Illumina HiSeq 2500 or Illumina MiSeq instruments to a median coverage of 750x. 62% of samples had ctDNA fraction above 2% of total cfDNA.

Note that "Dataset type" is erroneously listed as "Amplicon sequencing", because "Captured-based targeted sequencing" or "Hybridization-based targeted sequencing" were not available options in EGA at the time of submission.EGA dataset EGAD00001004208

本数据集包含从428名转移性前列腺癌男性患者中采集的712份血浆游离DNA（cell-free DNA，cfDNA）样本与428份白细胞样本的靶向测序数据。靶向捕获采用基于杂交的定制罗氏SeqCap EZ Choice试剂盒完成，该试剂盒专为捕获72个前列腺癌驱动基因的全部外显子区域而设计。游离DNA从10mL血液样本中提取得到。文库使用Illumina HiSeq 2500或Illumina MiSeq测序仪进行测序，中位测序深度达750×。62%的样本中，循环肿瘤DNA（circulating tumor DNA，ctDNA）占总游离DNA的比例高于2%。需注意，本数据集的「数据集类型」被错误标注为「扩增子测序」，原因是数据提交时，欧洲基因组表型档案馆（European Genome-phenome Archive，EGA）并未提供「基于捕获的靶向测序」或「基于杂交的靶向测序」选项。本数据集为EGA数据集EGAD00001004208