Comparison of peaks in Raman spectra of the whole heart, reduced or oxydized purified cytochrome c and purified oxy-or deoxymyoglobin.

*The same set of peaks we observe in Raman spectra of cardiomyocytes and isolated CM mitochondria;**Based on [11], [16], [17], [25] and our own observations;(a)— symmetric pyrrol half-ring vibrations of Mb heme (A1g/4 symmetry), sensitive to the Redox state of heme Fe and presence of O2;(b)and(c)— vibrations of heme methine-bridges (A1g and B1g/10 symmetry, respectively), sensitive to the spin state of heme Fe and diameter of the heme ring.Numbers indicate positions of peak maxima (cm−1). Arrows mark peaks whose intensity significantly increases in Raman spectra of the heart after SDT application, reduction of cytochrome c, or under binding or release of O2 from myoglobin. Cytochrome or Mb type indicated in bold font is the main contributer to the Raman scattering of the heart at the designated frequency shift.

*我们在心肌细胞（cardiomyocytes）与分离的CM线粒体（isolated CM mitochondria）的拉曼光谱（Raman spectra）中观测到了同一组特征峰；**基于文献[11]、[16]、[17]、[25]以及本研究团队的自主观测结果：
(a) — 肌红蛋白（Mb）血红素（heme）的对称吡咯半环振动，对应A1g/4对称群，对血红素铁（heme Fe）的氧化还原状态（Redox state）以及氧气（O₂）的存在状态敏感；
(b) 与(c) — 血红素次甲基桥的振动，分别对应A1g与B1g/10对称群，对血红素铁的自旋状态（spin state）以及血红素环的直径敏感；
文中标注的数字代表峰的最大位置（单位：cm⁻¹）。箭头所指的峰，其强度在心脏接受SDT处理、细胞色素c还原，或肌红蛋白结合/释放氧气时，在心脏拉曼光谱中显著升高。以粗体标注的细胞色素或肌红蛋白类型，为该指定频移（frequency shift）处心脏拉曼散射（Raman scattering）的主要贡献源。