Global Regulation of Alternative RNA Splicing by the SR-Rich Protein RBM39 CLIP-seq

RBM39 is extensively involved in alternative splicing of RNA and helps regulate transcript levels. RBM39 may modulate alternative splicing similarly to U2AF65 by either directly binding to RNA or recruiting other splicing factors, such as U2AF65. Overall design: Genome-wide RBM39-RNA interactions were mapped via cross-linking and immunoprecipitation coupled with deep sequencing (CLIP-Seq) in wild-type MCF-7 cells.

RBM39广泛参与RNA的可变剪接过程，并对转录本水平发挥调控作用。RBM39或可通过直接结合RNA或招募U2AF65等其他剪接因子，以与U2AF65相似的方式调控可变剪接。实验设计概况：在野生型MCF-7细胞中，研究人员通过交联免疫沉淀联合深度测序（CLIP-Seq）技术，绘制了全基因组范围内RBM39与RNA的互作图谱。