Effect of BTK and GSK3 inhibition on gene expression in primary peripheral human B cells

To investigate the BTK and GSK3 in the regulation of human B cell biology, we treated B cells from peripheral blood with either vehicle or BTK inhibitor or GSK3 in presence or absence of BCR cross-linking antibodies. We then performed gene expression profiling analysis using data obtained from RNA-seq of those samples at 24hrs. Overall design: Comparative gene expression profiling analysis of RNA-seq data for B cells treated with either BTK inhibitor or GSK3 inhibitor

为探究布鲁顿酪氨酸激酶（BTK）与糖原合成激酶3（GSK3）对人类B细胞生物学的调控作用，我们对外周血来源的B细胞开展如下处理：分别以溶媒对照、BTK抑制剂、GSK3抑制剂进行干预，并分别设置添加与不添加B细胞受体（BCR）交联抗体的实验组。随后，我们收集24小时后的样本，通过RNA测序（RNA-seq）获取数据，进行基因表达谱分析。
整体实验设计：对经BTK抑制剂或GSK3抑制剂处理的B细胞的RNA测序数据开展对比基因表达谱分析。