Genome-wide ZNF410 binding sites identified by CUT&RUN in HUDEP-2, human CD34+ HSPCs and MEL cells.

We examined the chromatin occupancy of ZNF410 by conducting CUT&RUN. We used an HA antibody to probe for epitope tagged ZNF410 in HUDEP-2 cells. We used a ZNF410 antibody to probe for endogenous ZNF410 in HUDEP-2 cells, human CD34+ hematopoietic stem and progenitor cell (HSPC) derived erythroid precursors and mouse erythroleukemia (MEL) cells. Genomic enrichment for ZNF410 binding was determined using IgG as the negative control.

本研究通过CUT&RUN技术检测ZNF410的染色质结合占有率。我们使用HA抗体对HUDEP-2细胞中表位标记的ZNF410进行靶标检测；同时采用ZNF410抗体，分别检测HUDEP-2细胞、人CD34+造血干祖细胞（hematopoietic stem and progenitor cell, HSPC）来源的红系前体细胞，以及小鼠红白血病（mouse erythroleukemia, MEL）细胞中的内源性ZNF410。以IgG作为阴性对照，分析ZNF410结合的基因组富集情况。