Members of an array of zinc finger proteins specify distinct Hox chromatin boundaries [ChIP-Seq1]

In this experiment, we sought to identify how the distribution of CTCF, MAZ, PATZ1, RAD21 change in WT and PATZ1 KO mouse embryonic stem cells (ESCs). Overall design: Examination of CTCF, MAZ, PATZ1, and RAD21 binding in embryonic stem cells using ChIP-seq, by status of WT and PATZ1 KO.

本实验旨在探究野生型（Wild Type, WT）与PATZ1基因敲除（Knock Out, KO）小鼠胚胎干细胞（embryonic stem cells, ESCs）中CTCF、MAZ、PATZ1、RAD21的分布变化情况。实验整体设计：采用染色质免疫共沉淀测序（Chromatin Immunoprecipitation sequencing, ChIP-seq）技术，基于野生型与PATZ1基因敲除的细胞状态，检测小鼠胚胎干细胞内CTCF、MAZ、PATZ1及RAD21的结合状况。