Susceptibility profile of Aedes aegypti from Santiago Island, Cabo Verde, to insecticides

This study aimed to assess the susceptibility of A. aegypti populations from Santiago, Cabo Verde to insecticides and identify the mechanisms of resistance. Samples of A. aegypti eggs were obtained at two different time periods (2012 and 2014), using ovitraps in different locations in Santiago Island to establish the parental population. F1 larvae were exposed to different concentrations of insecticides (Bacillus thuringiensis var israelensis (Bti), diflubenzuron and temephos) to estimate the lethal concentrations (LC90) and calculate the respective rate of resistance (RR90). Semi-field tests using temephos-ABATE® were performed to evaluate the persistence of the product. Bottle tests using female mosquitoes were carried out to determine the susceptibility to the adulticides malathion, cypermethrin and deltamethrin. Biochemical and molecular tests were performed to investigate the presence of metabolic resistance mechanisms, associated with the enzymes glutathione S-transferases (GSTs), esterases and mixed-function oxidases (MFO) and to detect mutations or alterations in the sodium channel and acetylcholinesterase genes. (MapVEu VBP0000361)

本研究旨在评估佛得角圣地亚哥岛埃及伊蚊（A. aegypti）种群对杀虫剂的敏感性，并鉴定其抗药性机制。研究于2012年与2014年两个不同时段，通过在圣地亚哥岛各点位布设诱卵器（ovitraps）采集埃及伊蚊卵，以此建立亲本种群。将F1幼虫暴露于不同浓度的杀虫剂——苏云金杆菌以色列亚种（Bacillus thuringiensis var israelensis, Bti）、除虫脲（diflubenzuron）与双硫磷（temephos）——中，以测定致死浓度LC90，并计算对应的抗药性倍数RR90。采用双硫磷制剂ABATE®开展半现场试验，以评估该产品的持效性。以成蚊为对象开展瓶法接触试验，明确其对马拉硫磷（malathion）、氯氰菊酯（cypermethrin）及溴氰菊酯（deltamethrin）这三类成虫杀虫剂的敏感性。通过生化与分子检测，探究与谷胱甘肽S-转移酶（glutathione S-transferases, GSTs）、酯酶及多功能氧化酶（mixed-function oxidases, MFOs）相关的代谢型抗药性机制的存在情况，并检测钠通道基因与乙酰胆碱酯酶基因的突变或变异。（数据集编号：MapVEu VBP0000361）