Taz protects aged hematopoietic stem cells from exhaustion [CITE-seq]

We report here the analysis of young and aged hematopoietic stem and progenitor cells (HSPCs) by transcriptional profiling (bulk RNA-Seq, CITE-Seq) and chromatin profiling (ATAC-Seq, scATAC-Seq). We use here a Clca3a1 as a surface marker to further fractionate HSPCs. HSPCs were sorted by flow cytometry. During the staining procedure for flow cytometry, the antibody cocktail also included antibodies labelled with oligos (antibody-derived tags =ADTs) which can later be analyzed by the 10x Chromium controller. Examination of transcriptional changes during aging of HSPCs.

本研究报道了通过转录组谱分析（bulk RNA-Seq、CITE-Seq）与染色质谱分析（ATAC-Seq、scATAC-Seq），对年轻与衰老造血干细胞和祖细胞（hematopoietic stem and progenitor cells，HSPCs）开展的系统性分析。本研究以Clca3a1作为表面标志物，对造血干细胞和祖细胞进行进一步分群。实验通过流式细胞术分选出目标细胞群，在流式染色流程中，抗体混合液还包含了带有寡核苷酸标记的抗体，即抗体衍生标签（antibody-derived tags，ADTs），后续可通过10x Chromium控制器对其进行检测分析。本研究同时对造血干细胞和祖细胞衰老过程中的转录组变化进行了考察。