AD-BXD [Ntg dataset 2]

Cg.5XFAD females (MMRRC Stock No #34848-JAX) were bred to males from BXD strains. The resulting F1 progeny were monitored throughout their lifepan to evaluate the effect of genetic background on cognitive and pathological traits. Samples here come from various Ntg-BXD lines at either 6 or 14 months of age. An earlier dataset of similar design (plus Non-transgenic littermates) was deposited as GSE101144. AD littermates of mice sampled here will be deposited as a separate GEO series. Overall design: 71 Ntg samples. For final by-strain analysis, samples were averaged into strain/age/genotype/sex groups (For example, all D2 6mo 5XFAD males were averaged for final by-strain analysis)

将Cg.5XFAD雌性小鼠（小鼠突变资源与研究中心库存编号：MMRRC Stock No #34848-JAX）与BXD品系雄性小鼠交配，所得F1子代在其整个生命周期内接受持续监测，以评估遗传背景对认知与病理表型的影响。本数据集的样本取自不同非转基因BXD品系（Ntg-BXD）小鼠，采样时小鼠年龄分别为6月龄或14月龄。一项设计相似的早期数据集（附带非转基因同窝对照小鼠）已以登录号GSE101144提交至基因表达综合数据库（Gene Expression Omnibus, GEO）。本研究中采样小鼠的阿尔茨海默病模型同窝转基因个体，将以独立的GEO数据集系列形式提交。

整体实验设计：共包含71份非转基因样本。在最终的品系水平分析中，所有样本将按品系/年龄/基因型/性别分组进行平均合并（例如，所有D2品系6月龄5XFAD雄性小鼠的样本将被合并平均，用于最终的品系水平分析）。