Identification of RRP1 binding sites using seCLIP-seq in IL-1ß stimulated RAW 264.7 cells.

How RRP1 control RNA processing and regulation in macrophage inflammation is important. Here is the identification for RRP1 binding sites by using the single-end enhanced CLIP(seCLIP) in RAW 264.7 cells. Overall design: To identify the interaction sites between RRP1 and its RNA targets in IL-1ß stimulated RAW264.7, seCLIP-seq analysis can be performed.RRP1在巨噬细胞炎症过程中调控RNA加工与表达的机制具有重要研究意义。本数据集通过在RAW 264.7细胞中运用单端增强交联免疫沉淀（single-end enhanced CLIP, seCLIP）技术，完成了RRP1结合位点的鉴定工作。整体实验设计：为鉴定经白细胞介素1β（IL-1β）刺激的RAW 264.7细胞内RRP1与其RNA靶标之间的互作位点，本研究开展了seCLIP-seq测序分析。