Evaluation of the effects of FGF8, Endothelin-1, and BMP4 (FEDB) on induced pluripotent stem cell (iPSC)-derived neural crest cells (NCCs) induced in two-dimensional (2D) conditions. Evaluation of the effects of FGF8, Endothelin-1, and BMP4 (FEDB) on induced pluripotent stem cell (iPSC)-derived neural crest cells (NCCs) induced in two-dimensional (2D) conditions

To evaluate the effects of FEDB on NCCs induced in 2D conditions, we sampled 2D cultured cells and cell aggregates during FEDB treatment and compared gene expression patterns. Overall design: RNA sequencing profiles of 1231A3 iPSC-derived NCC aggregates, NCC aggregates treated with FEDB, CD271high+ NCCs sorted from 2D induction protocols (2D NCC CD271high), and 2D NCC CD271high treated with FEDB in 2D culture (2D 271H+FEDB).

为评估FEDB对二维（2D）培养条件下诱导的神经嵴细胞（Neural Crest Cells，NCCs）的调控作用，本研究于FEDB处理过程中采集二维培养细胞及细胞聚集体样本，并对其基因表达模式开展对比分析。整体实验设计：对以下四类样本进行RNA测序分析：1231A3诱导多能干细胞（induced pluripotent stem cell，iPSC）衍生的NCC聚集体、经FEDB处理的NCC聚集体、从二维诱导方案中分选获得的CD271高阳性NCCs（2D NCC CD271high），以及在二维培养体系中经FEDB处理的2D CD271high NCCs（2D 271H+FEDB）。