H3K4 methylation buffers the effects of metabolic adaptation on replication initiation. H3K4 methylation buffers the effects of metabolic adaptation on replication initiation

In this study, we unravel a previously unknown function of H3K4 methylation (H3K4me; a universal marker of actively transcribed genes that also accumulates around replication origins) as a capacitor that masks the adverse effects of metabolic adaptation by facilitating initiation of replication. Overall design: 20 Samples of Rpb3, Mcm4 ChIP-Seq or ATAC-seq for S.cerevisiae in wild type and relevant mutant conditions.

本研究揭示了H3K4甲基化（H3K4me）此前未被发现的功能：作为一种缓冲因子，其可通过促进复制起始，掩盖代谢适应所引发的不良反应。H3K4me是活跃转录基因的通用标记，同时也会在复制起点区域富集。
实验整体设计：针对酿酒酵母（S.cerevisiae）在野生型及相关突变体条件下的20例样本，分别开展Rpb3、Mcm4的染色质免疫共沉淀测序（ChIP-Seq）或转座酶可及性测序（ATAC-seq）。