Additional file 1 of Overexpression of vesicle-associated membrane protein PttVAP27-17 as a tool to improve biomass production and the overall saccharification yields in Populus trees

Additional file 1: Fig. S1. Correlation matrix plot of data obtained from five platforms; GC–MS, LC–MS, Py–GC/MS, Proteomics, and Transcriptomics. Table S1. Monosaccharide composition of wood from wild-type (WT) and PttVAP27-17 overexpression lines of greenhouse-grown trees. Table S2. Relative abundance of metabolites identified by Pyrolysis–GC/MS analysis of wild-type and transgenic PttVAP27-17 overexpression lines grown in the greenhouse. Table S3. LC–MS metabolomic analysis of wild-type and PttVAP27-17 overexpression lines of greenhouse-grown trees. Table S4. GC–MS metabolomic analysis of wild-type and PttVAP27-17 overexpression lines of greenhouse-grown trees. Table S5. RNAseq analysis of wild-type and PttVAP27-17 overexpression lines of greenhouse-grown trees. Table S6. List of all peptides identified in the proteomic study. Table S7. List of all proteins identified in the proteomic study. Table S8. Genes co-expressed with PttVAP27-17. Table S9. Gene ontology (GO) analysis of transcripts (using tool REVIGO from Popgenie homepage popgenie.org) with statistically significant changes in the PttVAP27-17 overexpressing transgenic lines compared to the wild type of greenhouse-grown trees. Table S10. Gene ontology (GO) analysis of all proteins with statistically significant changes in the PttVAP27-17 overexpressing transgenic lines compared to the wild type of greenhouse-grown trees. Table S11. (a) Overexpression of PttVAP27-17 affects sugar yields of greenhouse-grown trees. (b) Overexpression of PttVAP27-17 affects sugar yields of field-grown trees.

附加文件1：补充图S1。五种检测平台（气相色谱-质谱（GC–MS）、液相色谱-质谱（LC–MS）、热解气相色谱-质谱（Py–GC/MS）、蛋白质组学（Proteomics）、转录组学（Transcriptomics））所得数据的相关性矩阵图。补充表S1。温室种植的野生型（wild-type，WT）与PttVAP27-17过表达株系木材的单糖组成。补充表S2。温室种植的野生型与转基因PttVAP27-17过表达株系的热解气相色谱-质谱（Py–GC/MS）分析鉴定得到的代谢物相对丰度。补充表S3。温室种植的野生型与PttVAP27-17过表达株系的液相色谱-质谱（LC–MS）代谢组学分析结果。补充表S4。温室种植的野生型与PttVAP27-17过表达株系的气相色谱-质谱（GC–MS）代谢组学分析结果。补充表S5。温室种植的野生型与PttVAP27-17过表达株系的RNA测序（RNAseq）分析结果。补充表S6。蛋白质组学研究中鉴定得到的全部肽段列表。补充表S7。蛋白质组学研究中鉴定得到的全部蛋白质列表。补充表S8。与PttVAP27-17共表达的基因列表。补充表S9。相较于温室种植的野生型株系，PttVAP27-17过表达转基因株系中存在统计学显著差异变化的转录本的基因本体（GO）分析结果，分析使用Popgenie官网（popgenie.org）提供的REVIGO工具完成。补充表S10。相较于温室种植的野生型株系，PttVAP27-17过表达转基因株系中存在统计学显著差异变化的全部蛋白质的基因本体（GO）分析结果。补充表S11。(a) PttVAP27-17过表达影响温室种植树木的糖产量；(b) PttVAP27-17过表达影响田间种植树木的糖产量。