Effect of 4-octyl itaconate on gene expression of dendritic cells

Here, we report that macrophage-derived itaconate exerts a significant suppressive effect on dendritic cell (DC) function. To delve deeper into the impact of itaconate on DCs, we treated bone marrow-derived dendritic cells with 4-OI, a derivative of itaconate, and conducted RNA-seq analysis. Gene-set enrichment analysis (GSEA) of 4-OI-treated DCs showed a general downregulation of innate immunity and immune-response pathways. Overall design: To further investigate the effect of itaconate on DCs, we performed RNA-seq analysis on bone marrow-derived dendritic cells (BMDCs) that were either incubated with IFN-? alone or co-treated with IFN-? and 4-OI. We then performed gene expression profiling analysis using the RNA-seq data obtained from BMDCs of IFN-? group and IFN-?+4-OI group. Comparative gene expression profiling analysis of RNA-seq data for BMDCs of IFN-? group and IFN-?+4-OI group.

本研究报道，巨噬细胞来源的衣康酸（itaconate）对树突状细胞（dendritic cell, DC）功能具有显著的抑制作用。为深入探究衣康酸对树突状细胞的影响，我们使用衣康酸衍生物4-OI处理骨髓来源的树突状细胞，并开展了RNA测序（RNA-seq）分析。对经4-OI处理的树突状细胞进行基因集富集分析（Gene-set enrichment analysis, GSEA）结果显示，天然免疫及免疫应答通路普遍出现下调。
实验整体设计：
为进一步探究衣康酸对树突状细胞的作用，我们对分别经单纯干扰素γ（interferon gamma, IFN-γ）孵育、以及经干扰素γ与4-OI联合处理的骨髓来源树突状细胞（bone marrow-derived dendritic cells, BMDCs）开展了RNA测序分析。随后，我们利用干扰素γ组与干扰素γ+4-OI组骨髓来源树突状细胞的RNA测序数据，进行了基因表达谱分析。针对干扰素γ组与干扰素γ+4-OI组骨髓来源树突状细胞的RNA测序数据，开展了对比基因表达谱分析。