Adipose Mesenchymal Cells-Derived Extracellular Vesicles Alleviate DOCA-salt-Induced Hypertension by Promoting Cardio-Renal Protection. Adipose Mesenchymal Cells-Derived Extracellular Vesicles Alleviate DOCA-salt-Induced Hypertension by Promoting Cardio-Renal Protection

By applying miRNA microarray profile of the kidney of DOCA-salt rats, we identified a selective miRNA signature associated with epithelial-mesenchymal transition (EMT) Overall design: Renal cortex was isolated from DOCA-salt rats treated for 8-week with deoxycorticosterone acetate and high salt diet in the presence or not of EVs and compared with nephrectomized animals (UNX) and SHAM animals

本研究通过分析醋酸脱氧皮质酮（deoxycorticosterone acetate, DOCA）盐诱导大鼠的肾脏miRNA微阵列谱（miRNA microarray profile），筛选得到与上皮间质转化（epithelial-mesenchymal transition, EMT）相关的特异性miRNA表达特征。实验设计：对接受醋酸脱氧皮质酮注射并辅以高盐饮食干预8周的DOCA盐诱导大鼠，设置是否给予细胞外囊泡（Extracellular Vesicles, EVs）处理的两组样本，并以肾切除（UNX）动物与假手术（SHAM）动物作为对照，对其分离得到的肾皮质组织进行比对分析。