SH1487351.10FU

UNITE provides a unified way for delimiting, identifying, communicating, and working with DNA-based Species Hypotheses (SH). All fungal ITS sequences in the international nucleotide sequence databases are clustered to approximately the species level by applying a set of dynamic distance values (<0.5 - 3.0%). All species hypotheses are given a unique, stable name in the form of a DOI, and their taxonomic and ecological annotations are verified through distributed, web-based third-party annotation efforts. SHs are connected to a taxon name and its classification as far as possible (phylum, class, order, etc.) by taking into account identifications for all sequences in the SH. An automatically or manually designated sequence is chosen to represent each such SH. These sequences are released (https://unite.ut.ee/repository.php) for use by the scientific community in, for example, local sequence similarity searches and next-generation sequencing analysis pipelines. The system and the data are updated automatically as the number of public fungal ITS sequences grows.

UNITE 提供了一套统一的方法，用于界定、识别、传播以及开展基于DNA的物种假说（Species Hypotheses，简称SH）相关工作。国际核酸序列数据库中的所有真菌内转录间隔区（Internal Transcribed Spacer，简称ITS）序列，通过应用一套动态距离阈值（0.5%~3.0%）被聚类至近似物种水平的分类单元。所有物种假说均被赋予唯一且稳定的数字对象唯一标识符（Digital Object Identifier，简称DOI）格式名称，其分类学与生态学注释信息将通过分布式网络第三方注释项目进行核验。各物种假说会尽可能结合该假说下所有序列的鉴定结果，与对应的分类单元名称及其分类层级（门、纲、目等）建立关联。研究人员将选取一条经自动或人工指定的代表序列，用以表征每个物种假说。此类代表序列已对外发布（https://unite.ut.ee/repository.php），可供科学界用于本地序列相似性检索、高通量测序分析流程等研究场景。随着公共真菌ITS序列数量的持续增长，该系统与配套数据集将自动完成更新。