Additional file 5: Figure S2. of Optimizing mesoderm progenitor selection and three-dimensional microniche culture allows highly efficient endothelial differentiation and ischemic tissue repair from human pluripotent stem cells
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3D microniche dramatically improved endothelial differentiation efficiency from hiPSCs. (a and b) Immunostaining showing CD31 expression in 2D (a) and 3D GM (b) on differentiated day 12 respectively. Scale bars: 100 μm. (c) Percentage of CD31+ cells on differentiated day 12 in 2D and 3D GM (n = 3, *** p
3D微龛(3D microniche)可显著提升人类诱导多能干细胞(human induced pluripotent stem cells, hiPSCs)向内皮细胞的分化效率。(a)与(b)分别为分化第12天时,2D体系(图a)与3D GM体系(图b)中CD31表达的免疫染色结果。比例尺:100 μm。(c) 分化第12天时,2D与3D GM体系中CD31阳性细胞的占比(n=3,*** p)
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2017-01-24



