Monocyte-derived IL-1 and IL-6 are differentially required for cytokine release syndrome and neurotoxicity by CAR-T cells.

Single-cell transcriptome profiling using a 3' droplet-based platform (Chromium,10x Genomics) of human CD45+ leukocytes isolated from leukemic HuSGM3 mice infused with CD19.28z CAR-T cells, two days after cytokine release syndrome (CRS) onset and 5 days later.

本数据集采用10x Genomics公司的Chromium系统（基于液滴的3'端单细胞转录组谱分析平台），针对两类时间点的样本完成单细胞转录组谱分析。所分析的样本为从输注了CD19.28z嵌合抗原受体T（CAR-T）细胞的白血病模型HuSGM3小鼠体内分离得到的人类CD45阳性白细胞，两类样本分别采集自细胞因子释放综合征（CRS）发作后2天，以及发作后再过5天的时间点。