The Impact of Acute Exposure to Cigarette Smoke on Airway Gene Expression. Homo sapiens

BACKGROUND: We have previously reported gene expression changes in the bronchial airway epithelium of active chronic smokers. In this study, we investigate the effects of Acute Smoke Exposure (ASE) from cigarettes on airway epithelial gene expression. METHODS: Bronchial airway epithelial cell brushings were collected via fiberoptic bronchoscopy from 63 individuals without recent exposure to cigarette smoke (> 2 days), at baseline and at 24 hours after smoking three cigarettes. RNA from these samples was profiled on Affymetrix Human Gene 1.0 ST microarrays. Differential gene expression was assessed using linear modeling and compared to previous smoking-related gene-expression signatures using Gene Set Enrichment Analysis (GSEA). RESULTS: We identified 91 genes differentially expressed 24-hours after exposure to three cigarettes (FDR 2 days), at baseline and at 24 hours after smoking three cigarettes. RNA from these samples was profiled on Affymetrix Human Gene 1.0 ST microarrays. Differential gene expression was assessed using linear modeling and compared to previous smoking-related gene-expression signatures using Gene Set Enrichment Analysis (GSEA).

【背景】我们此前曾报道过活动性慢性吸烟者支气管气道上皮的基因表达变化。本研究旨在探究香烟急性烟雾暴露（Acute Smoke Exposure, ASE）对气道上皮细胞基因表达的影响。
【方法】本研究通过纤维支气管镜，从63名近期无香烟烟雾暴露（≥2天）的受试者中，分别于基线状态以及吸烟3支后的24小时采集支气管气道上皮细胞刷检样本。采用Affymetrix人类基因1.0 ST微阵列对上述样本的RNA进行表达谱分析。通过线性模型评估差异基因表达情况，并借助基因集富集分析（Gene Set Enrichment Analysis, GSEA）与既往报道的吸烟相关基因表达特征进行比对。
【结果】本研究鉴定出暴露于3支香烟24小时后存在差异表达的91个基因（错误发现率<0.05，|log₂倍变化倍数|>1）。本研究对上述样本的RNA采用Affymetrix人类基因1.0 ST微阵列进行表达谱分析，并通过线性模型评估差异基因表达，借助基因集富集分析（GSEA）与既往吸烟相关基因表达特征进行比对。