AXMI277 NEMATODE TOXIN AND METHODS FOR ITS USE

Compositions and methods for conferring pesticidal activity to bacteria， plants， plant cells， tissues and seeds are provided. Compositions comprising a coding sequence for a toxin polypeptide are provided. The coding sequences can be used in DNA constructs or expression cassettes for transformation and expression in plants and bacteria. Compositions also comprise transformed bacteria， plants， plant cells， tissues， and seeds. In particular， isolated toxin nucleic acid molecules are provided. Additionally， amino acid sequences corresponding to the polynucleotides are encompassed， and antibodies specifically binding to those amino acid sequences. In particular， the present invention provides for isolated nucleic acid molecules comprising nucleotide sequences encoding the amino acid sequence shown in SEQ ID NO:2 or 3， or the nucleotide sequence set forth in SEQ ID NO:1， as well as variants and fragments thereof.

本发明提供了用于赋予细菌、植物、植物细胞、组织及种子杀虫活性的组合物与方法。本发明还提供了包含毒素多肽编码序列的组合物。该编码序列可应用于DNA构建体或表达盒，以在植物与细菌中实现转化与表达。所述组合物还涵盖经转化的细菌、植物、植物细胞、组织及种子。具体而言，本发明提供了分离的毒素核酸分子。此外，本发明涵盖与该多核苷酸对应的氨基酸序列，以及可特异性结合这些氨基酸序列的抗体。具体而言，本发明提供了分离的核酸分子，其包含编码SEQ ID NO:2或3所示氨基酸序列的核苷酸序列，或SEQ ID NO:1所载述的核苷酸序列，及其变体与片段。