Gene expression profile after injection of rhabdoviral recombinant protein NV [PBS vs. NV]. Oncorhynchus mykiss

Due to the common presence of a non-virion NV gene absent from other fish rhabdoviruses, NV-coding rhabdoviruses, such as the viral haemorrhagic septicemia (VHSV), were placed into a new Novirhabdovirus genus. Because conflicting results do exist on the importance of NV for VHSV trout pathogenicity and the NV function is still unclear, the effects of intraperitoneal injection of recombinant NV protein were studied by using home-designed immune-related microarrays from rainbow trout Oncorhynchus mykiss. Overall design: Trouts were separated in two groups. One group was injected with PBS and it was used as control group. Second group was injected with soluble VHSV non-virion (NV) recombinant protein

由于其他鱼类弹状病毒均未携带非病毒粒子NV基因（non-virion NV gene），携带NV基因的弹状病毒（如病毒性出血性败血症病毒（viral haemorrhagic septicemia, VHSV））被划归至新建立的诺拉弹状病毒属（Novirhabdovirus）。鉴于当前关于NV基因对VHSV感染虹鳟致病力重要性的研究结果存在分歧，且NV蛋白的功能仍未明确，本研究采用自主研发的虹鳟（Oncorhynchus mykiss）免疫相关基因芯片，探究了腹腔注射重组NV蛋白的相关效应。实验设计概述：将虹鳟分为两组，一组注射磷酸盐缓冲液（PBS）作为对照组，另一组注射可溶性VHSV非病毒粒子（NV）重组蛋白。