Transcriptome of new DGCR8_KO mouse embryonic stem cells generated by paired CRISPR/Cas9 approach. Mus musculus

Purpose: Next-generation sequencing (NGS) has revolutionized systems-based analysis of cellular pathways. The goals of this study was to obtain the trasncriptome of DGCR8_KO mESCs to compare it with the transcriptome of WT mESCs (deposit separately). Overall design: mRNA profiles of DGCR8_KO mouse embryonic stem cells were generated by deep sequencing, in duplicate, using Illumina HiSeq2000.

研究目的：下一代测序（Next-generation sequencing, NGS）已彻底革新了细胞通路的系统生物学分析。本研究旨在获取DGCR8基因敲除小鼠胚胎干细胞（DGCR8_KO mESCs）的转录组，以与野生型（Wild Type, WT）小鼠胚胎干细胞的转录组进行对比（后者将单独提交）。整体实验设计：采用Illumina HiSeq2000测序平台对DGCR8基因敲除小鼠胚胎干细胞进行双重复深度测序，以获取其mRNA表达谱。