CIL:9197, Homo sapiens, permanent cell line cell, epithelial cell. In Cell Image Library

Sialyl Transferase-EGFP transfected HeLa cells demonstrating the redistribution of the Golgi Apparatus to the Endoplasmic Reticulum upon treatment with Brefeldin A (BFA).
Images were collected as a 4D time series (z-stack over time). z-stacks of 48 individual timepoints are included in this collection of files as well as the movie SiEGFP_BFA.avi, which is the maximum projection of the z-stacks at the individual time points.

BFA was added according to the following protocol:
Timepoint1: Before addition of BFA 0seconds (8 Z-slices at 1 um apart)
Timepoint2: After addition of BFA 130seconds (8 Z-slices at 1 um apart)
Timepoint3: 238 seconds (11 Z-slices at 1 um apart)
Timepoint3 thru Timepoint48: Images captured every 5 seconds (11 Z-slices at 1 um apart at each time point)

Images were collected on a Carl Zeiss spinning disk confocal with a 63X 1.4 NA objective with 250 ms exposures at 5 s intervals.

转染了唾液酸转移酶-增强型绿色荧光蛋白（Sialyl Transferase-EGFP）的海拉细胞（HeLa cells），经布雷菲德菌素A（Brefeldin A，BFA）处理后，可观测到高尔基体（Golgi Apparatus）向内质网（Endoplasmic Reticulum）发生重定位。
本数据集采集得到四维时间序列（4D time series）图像，即随时间变化的z轴堆叠（z-stack）。本文件集中收录了48个独立时间点的z轴堆叠图像，同时附带视频文件SiEGFP_BFA.avi，该视频为各时间点z轴堆叠的最大投影（maximum projection）结果。
布雷菲德菌素A的添加遵循如下实验流程：
时间点1：布雷菲德菌素A添加前0秒（采集8层Z切片，层间距1 μm）
时间点2：布雷菲德菌素A添加后130秒（采集8层Z切片，层间距1 μm）
时间点3：处理后238秒（采集11层Z切片，层间距1 μm）
时间点3至时间点48：每5秒采集一次图像（每个时间点均采集11层Z切片，层间距1 μm）
图像采集使用卡尔蔡司（Carl Zeiss）转盘共聚焦显微镜（spinning disk confocal），搭配63× 1.4数值孔径（NA）物镜，曝光时长250 ms，采集间隔为5秒。