Gene expression profile at single cell level of skin cells isolated from db/db mice

The Janus liposozyme robustly eradicates infections and rapidly promotes wound closure and re-epithelialization on diabetic skin wound infected with methicillin-resistant S. aureus (MRSA). We used single cell RNA sequencing(scRNA-seq) to deep analyse local immune homeostasis manipulated by Janus on skin cells obtained from db/db mice . Overall design: db/db mice were infected with methicillin-resistant S. aureus (MRSA) and then were randomly divided into two groups: (i) PBS as control; (ii) TSeL with light irradiation (TSeL+L). Subsequently, skin tissues were harvested on day15 and further dissociated into single cells without separating dermis and epidermis. And skin cells were analyzed using scRNA-seq.

Janus脂质酶（Janus liposozyme）可强效根除感染，在耐甲氧西林金黄色葡萄球菌（methicillin-resistant S. aureus, MRSA）感染的糖尿病皮肤创面中快速促进创面闭合与再上皮化。我们采用单细胞RNA测序（single cell RNA sequencing, scRNA-seq），对从db/db小鼠获取的皮肤细胞中Janus所调控的局部免疫稳态开展深度分析。实验总体设计：将db/db小鼠先以耐甲氧西林金黄色葡萄球菌（MRSA）感染，随后随机分为两组：(i) 磷酸盐缓冲液（PBS）对照组；(ii) 光辐照TSeL组（TSeL+L）。后续于第15天收取皮肤组织，在未分离真皮与表皮的情况下将其解离为单细胞，随后通过scRNA-seq对皮肤细胞进行分析。