Transcriptomic analysis of primary human T cells and murine RAW 264.7 macrophages 3D cultured in different collagen densities

The density of the extracellular matrix has a dramatic impact on the gene expression profile of T cells and macrophages. This may reflect impaired immune response of T cells and macrophages in solid tumors. Quantification of RNA expression in human PBMCs enriched for T cells through the selection of non-adhenernt cells and a murine macrophage cell line (RAW 264.7) cultured in different densities of extracellular matrix (Type I collagen). T cells were isolated from PBMCs

细胞外基质（extracellular matrix）的密度对T细胞和巨噬细胞的基因表达谱具有显著影响，这或许反映了实体瘤内T细胞与巨噬细胞的免疫应答受损状况。本数据集针对两类样本开展RNA表达定量分析：其一为通过分选非黏附细胞富集T细胞的人外周血单个核细胞（PBMCs），其二为在不同密度的Ⅰ型胶原蛋白细胞外基质中培养的小鼠巨噬细胞系RAW 264.7，其中T细胞均分离自外周血单个核细胞。