Data_Sheet_1_Multisite Dopamine Sensing With Femtomolar Resolution Using a CMOS Enabled Aptasensor Chip.docx

Many biomarkers including neurotransmitters are found in external body fluids, such as sweat or saliva, but at lower titration levels than they are present in blood. Efficient detection of such biomarkers thus requires, on the one hand, to use techniques offering high sensitivity, and, on the other hand, to use a miniaturized format to carry out diagnostics in a minimally invasive way. Here, we present the hybrid integration of bottom-up silicon-nanowire Schottky-junction FETs (SiNW SJ-FETs) with complementary-metal–oxide–semiconductor (CMOS) readout and amplification electronics to establish a robust biosensing platform with 32 × 32 aptasensor measurement sites at a 100 μm pitch. The applied hetero-junctions yield a selective biomolecular detection down to femtomolar concentrations. Selective and multi-site detection of dopamine is demonstrated at an outstanding sensitivity of ∼1 V/fM. The integrated platform offers great potential for detecting biomarkers at high dilution levels and could be applied, for example, to diagnosing neurodegenerative diseases or monitoring therapy progress based on patient samples, such as tear liquid, saliva, or eccrine sweat.

诸多生物标志物（biomarkers）包括神经递质（neurotransmitters）可在汗液、唾液等体外体液中被检出，但其浓度水平远低于血液中的含量。因此，对此类生物标志物实现高效检测，一方面需要采用具备高灵敏度的技术，另一方面则需采用微型化检测形式，以开展微创诊断。本文报道了自下而上制备的硅纳米线肖特基结场效应晶体管（SiNW SJ-FETs）与互补金属氧化物半导体（CMOS）读出放大电子电路的混合集成方案，以此构建出一款拥有32×32个适体传感器检测位点、间距为100μm的稳健生物传感平台。所采用的异质结可实现低至飞摩尔级浓度的选择性生物分子检测。实验证实，该平台可对多巴胺实现选择性多位点检测，灵敏度高达约1 V/fM。这款集成平台在高稀释度样本的生物标志物检测中具备巨大应用潜力，可应用于基于泪液、唾液或外分泌汗液等患者样本的神经退行性疾病诊断，或是治疗进程监测等场景。