Transcription profiling by array of CREM-knockout mice

To assess a potential role of transcription factor CREM in the long-term detrimental effects of beta1-adrenoceptor overexpression, four mouse lines were generated and studied: wild-type mice (WT), Crem-normal beta1AR-transgenic mice (beta1ARTG), Crem-deficient non-transgenic mice (Crem-/-) and Crem-deficient beta1AR-transgenic mice (beta1ARTG/Crem-/-). We focused on genes up- or down-regulated in transgenic mice due to the lacking of CREM (beta1ARTG/Crem-/- vs. beta1ARTG).为评估转录因子CREM（transcription factor CREM）在β1肾上腺素能受体（beta1-adrenoceptor）过表达所介导的长期有害效应中的潜在作用，本研究构建并分析了四组小鼠品系：野生型小鼠（WT）、CREM正常的β1肾上腺素能受体转基因小鼠（beta1ARTG）、CREM敲除非转基因小鼠（Crem-/-）以及CREM敲除β1肾上腺素能受体转基因小鼠（beta1ARTG/Crem-/-）。本研究聚焦于在转基因小鼠中因CREM缺失而出现上调或下调的基因，以beta1ARTG/Crem-/-与beta1ARTG作为对照比较组进行分析。