RNA transcriptome sequencing of Skin Cutaneous Melanoma cell line A375 and SK-MEL-28 treated with siNC or siLINC01291

To figure out the role of long non-coding RNA LINC01291 in Skin Cutaneous Melanoma and the underlying mechanism, RNA transcriptome sequencing was performed to globally characterize LINC01291-regulated transcriptome changes. LINC01291 knockdown by siRNA in melanoma cells. A375 and SK-MEL-28 cells treated with siNC served as control. Overall design: Skin Cutaneous Melanoma cell line A375 and SK-MEL-28 treated with siNC or siLINC01291

为阐明长链非编码RNA（long non-coding RNA）LINC01291在皮肤黑色素瘤（Skin Cutaneous Melanoma）中的作用及其潜在分子机制，本研究通过RNA转录组测序（RNA transcriptome sequencing）全局表征受LINC01291调控的转录组变化。实验通过小干扰RNA（small interfering RNA, siRNA）敲低黑色素瘤细胞中的LINC01291，以转染阴性对照小干扰RNA（siNC）的A375与SK-MEL-28细胞作为对照组。实验设计概述：使用siNC或siLINC01291处理皮肤黑色素瘤细胞系A375及SK-MEL-28。