DNA sequencing of Cannabis specimens accessioned in herbarium ISTE

Humans have domesticated Cannabis for a variety of uses and mediated its dispersal to every continent on Earth. This project provides DNA sequencing data for specimens accessioned in the ISTE herbarium only and is organized under an umbrella project ("Population Dynamics in Cannabaceae") that includes sampling from several institutions around the world. The original herbarium specimens have been annotated with a tissue sample collection code. The original herbarium labels were translated from a variety of languages to English. Tissue samples have been annotated with metadata available from the herbarium specimens, including: collector, collection date, and collection coordinates. Collection coordinates were determined directly when available; most however, were georeferenced from the herbarium specimen collection locality using maps pertinent to the collection date. DNA libraries were prepared using protocols designed specifically for degraded plant tissue. The goals of this project include: inferring the relationships of Cannabis populations in their native range, timing historical human-mediated dispersal events, timing selective sweeps associated with domestication traits, understanding sex-chromosome dynamics during domestication, and establishing a geo-genetic database that can be used to infer the ancestry of genomic tracts in modern, perhaps admixed, Cannabis individuals. These data are being released under the tenants of the Fort Lauderdale Agreement.

人类已将大麻属（Cannabis）植物驯化以满足多种用途，并推动其传播至全球各大陆。本项目仅针对收录于ISTE植物标本馆（ISTE herbarium）的标本提供DNA测序数据，隶属于统筹项目“大麻科种群动态（Population Dynamics in Cannabaceae）”，该项目的采样覆盖全球多家科研机构。原始植物标本均已标注组织样本采集编码；原始标本馆标签已从多种语言译为英文。组织样本已附带来自标本的元数据，包括采集者、采集日期及采集坐标。采集坐标可直接获取时直接记录；多数情况下则依据标本采集地，结合采集当季相关地图完成地理配准。DNA文库的构建采用专为降解植物组织开发的实验方案。本项目的研究目标包括：推断大麻属本土种群的演化关系、确定人类介导的历史传播事件的时间节点、解析与驯化性状相关的选择性清除发生时间、阐明驯化过程中性染色体的动态变化，以及构建地理遗传数据库，用于推断现代（可能为混合起源的）大麻个体基因组片段的祖先来源。本项目数据将依据劳德代尔堡协议（Fort Lauderdale Agreement）的相关条款公开共享。