Single-cell transcriptomic reveals heterogeneous feature of myeloid-derived suppressor cells in newborns

In order to gain deep insights into the molecular characteristics and heterogeneity of MDSCs in human newborns, low-density CD11b+HLA-DR-/low immature myeloid cells from the peripheral blood of term infants, preterm infants, and adults control were subjected to single-cell RNA sequencing (scRNA-seq). Overall design: Peripheral blood samples were collected from full-term, preterm and adult controls. Single-cell suspensions of peripheral blood mononuclear cells (PBMCs) were obtained by centrifuging human blood samples on a Ficoll gradient. The strategy for MDSCs was CD11b+HLA-DR-/lo. Live immature myeloid cell in RPMI1640 supplemented with 20% FBS were sorted in a BD FACSAria III cell sorter (BD Biosciences).

为深入探究人类新生儿髓系来源抑制细胞（myeloid-derived suppressor cells, MDSCs）的分子特征与异质性，本研究对足月儿、早产儿及成人对照组外周血来源的低密度CD11b阳性、HLA-DR阴性或低表达的未成熟髓系细胞开展了单细胞RNA测序（scRNA-seq）。
实验设计：采集足月儿、早产儿及成人对照组的外周血样本。通过Ficoll密度梯度离心法分离获得外周血单个核细胞（PBMCs）的单细胞悬液。本研究采用CD11b阳性、HLA-DR阴性或低表达的策略分选髓系来源抑制细胞（MDSCs）。将活的未成熟髓系细胞置于添加20%胎牛血清（FBS）的RPMI1640培养基中，随后使用BD FACSAria III细胞分选仪（BD Biosciences）完成分选。