The Resistome: A Comprehensive Database of Escherichia coli Resistance Phenotypes

The microbial ability to resist stressful environmental conditions and chemical inhibitors is of great industrial and medical interest. Much of the data related to mutation-based stress resistance, however, is scattered through the academic literature, making it difficult to apply systematic analyses to this wealth of information. To address this issue, we introduce the Resistome database: a literature-curated collection of Escherichia coli genotypes–phenotypes containing over 5,000 mutants that resist hundreds of compounds and environmental conditions. We use the Resistome to understand our current state of knowledge regarding resistance and to detect potential synergy or antagonism between resistance phenotypes. Our data set represents one of the most comprehensive collections of genomic data related to resistance currently available. Future development will focus on the construction of a combined genomic–transcriptomic–proteomic framework for understanding E. coli’s resistance biology. The Resistome can be downloaded at https://bitbucket.org/jdwinkler/resistome_release/overview.

微生物抵抗胁迫环境条件与化学抑制剂的能力具有重要的工业与医学研究价值。然而，绝大多数基于突变的抗逆性相关数据分散于各类学术文献中，导致难以对这些海量信息开展系统性分析。为解决这一问题，我们推出了抗组数据库（Resistome）：一款经文献整理收录的大肠杆菌（Escherichia coli）基因型-表型数据集，涵盖超过5000株可抵御数百种化合物与环境胁迫条件的突变体。我们利用该数据库来厘清当前学界对抗逆性的认知现状，并检测抗逆表型间潜在的协同或拮抗作用。本数据集是目前公开可得的最全面的抗逆性相关基因组数据集之一。未来的开发工作将聚焦于构建基因组-转录组-蛋白质组整合分析框架，以解析大肠杆菌的抗逆生物学机制。用户可通过https://bitbucket.org/jdwinkler/resistome_release/overview 下载该数据库。