Altered mRNA expression of cultured dorsal root ganglia neurons at different times of rat

To explored the altered genes during the axon regeneration of rat dorsal root ganglia neurons, we utilized Gene Expression Array to find out the altered expression of genes in cultured DRG neurons at different times. When we digest the cells from rat DRGs with enzymes, we only get the cell bodies of neurons without axons. During the axons re-growth after the neurons planted, it must be amount of genes expression will changed. However, we still not fully understand which genes will changed and the function of these genes. In addition, this cell model could mimic the regeration of sciatic nerve after injure in vivo, which is still a challenge in clinical. At 0h, 1.5h, 3h, 6h, 12h, 18h, 24h, 30h, 36h after neurons planted, the total RNA was isolated from these cells for Gene Expression Array. Three independent experiments were performed at each time point.

为探索大鼠背根神经节（Dorsal Root Ganglia, DRG）神经元轴突再生过程中的差异表达基因，我们采用基因表达芯片（Gene Expression Array）技术，对不同培养时间的体外培养DRG神经元中的基因表达变化进行检测。当通过酶解法解离大鼠DRG组织时，仅能获取神经元胞体而无法保留轴突。神经元接种后轴突重新生长的过程中，必然伴随大量基因表达的改变，但目前我们尚未完全明确哪些基因会发生表达变化，以及这些基因的具体功能。此外，该细胞模型可模拟体内坐骨神经损伤后的再生过程，而这一过程在临床治疗中仍存在诸多挑战。在神经元接种后的0h、1.5h、3h、6h、12h、18h、24h、30h、36h这9个时间点，我们分别收集细胞并提取总RNA，用于基因表达芯片检测。每个时间点均开展3次独立重复实验。