Transcriptional profiling of pig striatum after deep hypothermic cardiac arrest. Transcriptional profiling of pig striatum after deep hypothermic cardiac arrest

The goal of the study was to identify the gene changes that occur in the striatum of neonatal piglets exposed to cardiopulmonary bypass (CPB) with deep hypothermic cardiac arrest (DHCA) then recovered for 6h. To accomplish our goal, mRNA-seq was performed on RNA isolated from neonatal pigs exposed to either DHCA or sham conditions. Overall design: RNA samples were isolated from the striatum from the brains of 6 neonatal piglets exposed to sham conditions were used as the controls; while samples from 6 piglets exposed to DHCA of 18o for 30 min then recovered for 6h were the experimental group.

本研究旨在鉴定暴露于深低温停循环（deep hypothermic cardiac arrest, DHCA）联合体外循环（cardiopulmonary bypass, CPB）并恢复6小时的新生仔猪纹状体中发生的基因表达变化。为实现该研究目标，我们对分别暴露于深低温停循环或假手术条件的新生仔猪分离获得的RNA开展了mRNA测序（mRNA-seq）。
总体实验设计：以6只假手术组新生仔猪脑组织纹状体提取的RNA样本作为对照组；以6只经18℃深低温停循环30分钟、随后恢复6小时的仔猪样本作为实验组。