Tissue overgrowth induced by coexpression of the progenitor genes hth/MEIS1 and tsh/TSHZ results from an imbalance in the estrogen response pathway in Drosophila [FAIRE-Seq]. Drosophila melanogaster

Analysis of third instar eye-antennal Drosophila imaginal discs with forced expression of hth, tsh or hth+tsh in the eye using an eye-specific GAL4 driver (optix2.3-GAL4). Forced maintenance of hth+tsh expression (but not of any of the two alone) results in overgrowth and aberrant cell differentiation. Results provide insight into new targets of hth+tsh. Overall design: RNA-seq gene expression profiling across Drosophila 3rd instar larval wild type eye-antennal discs and genetic perturbations of tsh, hth and tsh-hth together.

本研究利用眼特异性GAL4驱动子（eye-specific GAL4 driver，optix2.3-GAL4），对在眼部强制过表达hth、tsh或hth+tsh的果蝇三龄眼触角成虫盘开展分析。仅持续维持hth与tsh的共过表达（二者单独过表达均无该效应）会引发组织过度增生与异常细胞分化。本研究结果可为解析hth与tsh协同调控的新靶标提供研究线索。实验整体设计：对果蝇三龄幼虫的野生型眼触角成虫盘，以及分别遗传扰动tsh、hth与同时共扰动tsh-hth的样本进行RNA-seq基因表达谱分析。