Genome-wide maps of chromatin state in CTRLcas9 and SCARB2cas9 HepG2 cells.

We report the application of single-molecule-based sequencing technology for high-throughput profiling of c-Myc with core promoters of its target genes in mammalian cells. We performed ChIP sequencing with antibody to total c-Myc with core promoters of its target genes in CTRLcas9 and SCARB2cas9 group. Inspection of multiple individual traces and global analyses showed that SCARB2cas9 globally reduced association of c-Myc with core promoters of its target genes. Overall design: we precipitated chromatin with tag antibody to CTRLcas9 and SCARB2cas9 HepG2 cells, and sequenced the precipitated material. SCARB2cas9 reduced association of c-Myc with core promoters of its target genes

本研究报道了基于单分子测序技术（single-molecule-based sequencing technology）在哺乳动物细胞中对c-Myc及其靶基因核心启动子进行高通量谱分析的应用。我们在CTRLcas9组与SCARB2cas9组中，使用抗总c-Myc抗体针对其靶基因的核心启动子开展了染色质免疫沉淀测序（ChIP Sequencing）。对多条独立测序轨迹的检视与全局分析结果显示，SCARB2cas9可全局性降低c-Myc与其靶基因核心启动子的结合水平。整体实验设计：我们使用靶向转染CTRLcas9与SCARB2cas9的HepG2细胞的标签抗体沉淀染色质，并对沉淀得到的物质进行测序。SCARB2cas9可降低c-Myc与其靶基因核心启动子的结合能力。