Transcriptomic analysis of the effect of CT16 (PAGE5) expression in WM-266-4 melanoma cells. Homo sapiens

The cellular gene expression profiles were investigated in CT16 (PAGE5) negative WM-266-4 melanoma cells as well as in the WM-266-4 cells expressing transfected CT16 cDNA. Overall design: Total RNA obtained from three individual cultures of WM-266-4 cells stably transfected with CT16 cDNA- containing plasmid and from three individual cultures of WM-266-4 cells stably transfected with intact vector.

本研究针对CT16（PAGE5）阴性的WM-266-4黑色素瘤细胞，以及转染CT16互补脱氧核糖核酸（complementary DNA，cDNA）的WM-266-4细胞，开展了细胞基因表达谱分析。实验总体设计：实验所需总RNA分别提取自3份独立培养的、稳定转染含CT16 cDNA质粒的WM-266-4细胞，以及3份独立培养的、稳定转染空载体质粒的WM-266-4细胞。