Additional file 1 of ExonSurfer: a web-tool to design primers at exon–exon junctions

Additional file 1: Supplementary Table 1 displays primer designs generated using ExonSurfer, including gene details, efficiency percentages, tested cell lines, primer sequences, amplicon characteristics, melting temperatures, alignment scores, and transcript detection outcomes. Primer efficiency is calculated by the equation E = 10-1/slope, with an efficiency of 2 indicating that for each cycle the amount of product doubles.

附加文件1：补充表1展示了使用ExonSurfer生成的引物设计方案，包含基因详情、扩增效率百分比、已测试细胞系、引物序列、扩增子（amplicon）特性、解链温度、比对得分以及转录本检测结果。引物效率通过公式E = 10^(-1/斜率)计算得出，当效率值为2时，意味着每经过一个循环，产物的量将翻倍。