The Effect of TLR2 Activation on Transcriptome of IFN-induced Microglia. The Effect of TLR2 Activation on Transcriptome of IFN-induced Microglia

In murine glioma models, TLR2 is selectively upregulated in glioma-associated microglia. We found that TLR2 activation led to down-regulation of MHC-II in microglia. Therefore, we examined the effect of TLR2 activation on IFN-gamma-induced microglial transcriptome by in vitro culture of murine adult microglia. In the study, we confirmed that TLR2 activation led to Ciita shutdown and inhibition of MHC-II related genes, which also revealed the role of TLR2 in inducing APC disability and tumor immune escape. In addition, this work can be used to reveal the transcriptional characteristics of murine microglia during IFN-gamma and TLR2 activation. Overall design: Examination of transcriptional characteristics in murine adult microglia under TLR2 agonist and IFN-gamma treatments.

在小鼠胶质瘤模型中，Toll样受体2（Toll-like receptor 2, TLR2）在胶质瘤相关小胶质细胞中呈选择性上调表达。本研究发现，TLR2激活可导致小胶质细胞的主要组织相容性复合体II类（Major Histocompatibility Complex II, MHC-II）表达下调。据此，我们通过体外培养小鼠成年小胶质细胞，探究了TLR2激活对干扰素γ（Interferon-gamma, IFN-γ）诱导的小胶质细胞转录组的影响。本研究证实，TLR2激活可引发Ciita基因沉默并抑制MHC-II相关基因的表达，同时揭示了TLR2在诱导抗原呈递细胞（Antigen-Presenting Cell, APC）功能缺陷及肿瘤免疫逃逸中的作用。此外，本研究可用于揭示小鼠小胶质细胞在干扰素γ与TLR2激活过程中的转录特征。实验整体设计：检测经TLR2激动剂与干扰素γ处理的小鼠成年小胶质细胞的转录特征。