Variant <i>in vivo</i> activity classification.

Variant activities in vivo were classified based on their iSAT, iT and cell doubling rates. The variants showing growth rates within error of the wild type were grouped under ‘No defect’. Variants with defects in growth, iSAT and iT rates were considered to have a translation defect, as iT reactions are initiated after ribosomes have been assembled beforehand. Variants displaying defects in growth and iSAT rates but with iT within error of the wild type were considered to have a ribosome biogenesis defect. Finally, two variants display iSAT rates slightly below wild type, but no growth or iT rate defects, and have been classified as undetermined. (XLSX)体内变异体的活性基于其iSAT、iT及细胞倍增速率进行分类。生长速率处于野生型（wild type）误差范围内的变异体被归为‘无缺陷’组。生长、iSAT与iT速率存在缺陷的变异体被判定为存在翻译缺陷，因iT反应是在核糖体预先组装完成后才启动的。仅生长与iSAT速率存在缺陷，但iT速率处于野生型误差范围内的变异体，被判定为存在核糖体生物发生缺陷。最后，有2株变异体的iSAT速率略低于野生型，但无生长或iT速率缺陷，因此被归为‘未确定’组。 (XLSX)