Effect of PPAR Gamma Agonist Rosiglitazone on Carotid Artery Gene Expression after Knockout of Retinol Binding Protein 7 (RBP7). Mus musculus

To examine the role of retinol binding protein 7 (RBP7) in PPAR gamma mediated regulation of target gene expression in the carotid artery, RNA-Seq was used to quantitate gene expression in carotid artery from both wild-type and RBP7 knockout mice after ligand-mediated activation of PPAR gamma with Rosiglitazone. Overall design: Carotid artery were removed from wild-type (WT) and RBP7 knockout (KO) mice and treated with either Rosliglitazone (ROSI, 10 uM) or vehicle DMSO (CONT) for 24 hrs.

为探究视黄醇结合蛋白7（retinol binding protein 7, RBP7）在过氧化物酶体增殖物激活受体γ（PPAR gamma）介导的颈动脉靶基因表达调控中的作用，本研究采用RNA测序（RNA-Seq）技术，对经罗格列酮（Rosiglitazone）配体激活PPARγ后的野生型与RBP7敲除小鼠的颈动脉组织开展基因表达定量分析。
实验设计概要：从野生型（WT）及RBP7敲除（KO）小鼠体内分离颈动脉组织，分别以10 μM罗格列酮（ROSI）或对照溶剂二甲基亚砜（DMSO，CONT）处理24小时。