Small RNA sequencing of Rice Efficient and Non-Efficient Genotypes to determine uptake of Nitrogen

4 rice genotypes were sequenced for nitrogen usage under low and high nitrogen conditions to determine the uptake, and later combine with RNASeq data to figure which genes were modulated The datasets were processed to remove adapters which are a trademark of smallRNA sequencing datasets; remove of the other ncRNA-like sequences; then perform quantification of known and identify denovo miRNAs, using Oryza sativa japonica as reference.

本研究对4个水稻基因型在低氮与高氮条件下开展测序，以解析其氮素吸收特性并探究氮素利用特征；后续结合RNA测序（RNASeq）数据，筛选受调控的基因。该数据集经以下预处理流程：首先去除小RNA测序数据集标志性的接头序列，移除其他类非编码RNA（ncRNA）序列；随后以粳稻（Oryza sativa japonica）为参考基因组，对已知微小RNA（miRNA）进行定量分析，并鉴定从头预测的miRNA（denovo miRNAs）。