Data_Sheet_1_Effect of Two Different Sugarcane Cultivars on Rhizosphere Bacterial Communities of Sugarcane and Soybean Upon Intercropping.PDF

Intercropping of soybean and sugarcane is an important strategy to promote sustainable development of the sugarcane industry. In fact, our understanding of the interaction between the rhizosphere and bacterial communities in the intercropping system is still evolving; particularly, the influence of different sugarcane varieties on rhizosphere bacterial communities in the intercropping process with soybean, still needs further research. Here, we evaluated the response of sugarcane varieties ZZ1 and ZZ9 to the root bacterial community during intercropping with soybean. We found that when ZZ9 was intercropped with soybean, the bacterial diversity increased significantly as compared to that when ZZ1 was used. ZZ9 played a major role in changing the bacterial environment of the root system by affecting the diversity of rhizosphere bacteria, forming a rhizosphere environment more conducive to the growth of sugarcane. In addition, our study found that ZZ1 and ZZ9 had differed significantly in their utilization of nutrients. For example, nutrients were affected by different functional genes in processes such as denitrification, P-uptake and transport, inorganic P-solubilization, and organic P-mineralization. These results are significant in terms of providing guidance to the sugarcane industry, particularly for the intercropping of sugarcane and soybean in Guangxi, China.

大豆与甘蔗间作是推动甘蔗产业可持续发展的重要策略。事实上，我们对间作系统中根际（rhizosphere）与细菌群落间的互作关系的认知仍在不断深化；尤为关键的是，不同甘蔗品种在与大豆间作过程中对根际细菌群落的影响机制，尚有待进一步研究。本研究评估了甘蔗品种ZZ1与ZZ9在与大豆间作时，其根系细菌群落的响应特征。研究发现，相较于与大豆间作的ZZ1品种，ZZ9与大豆间作时的细菌群落多样性显著提升。ZZ9可通过调控根际细菌多样性，显著改变根系细菌微环境，进而形成更适配甘蔗生长的根际微环境。此外，本研究发现ZZ1与ZZ9在养分利用方面存在显著差异：例如，在反硝化作用（denitrification）、磷吸收与转运（P-uptake and transport）、无机磷溶解（inorganic P-solubilization）以及有机磷矿化（organic P-mineralization）等过程中，养分代谢会受到不同功能基因的调控。本研究结果可为甘蔗产业发展提供重要指导，尤其可为中国广西地区的甘蔗-大豆间作实践提供理论支撑。