Effect of Various Concentrations of an Anise Seed Powder (Pimpinella Anisum L.) Supplement on Selected Hematological and Biochemical Parameters of Broiler Chickens

ABSTRACT The objective of the study was to investigate the influence of anise seed (Pimpinella anisum L.) powder supplemented to the drinking water on selected blood parameters of broilers. In total, 360 one-day-old Hubbard Classic chicks were randomly divided into four groups with three replicates of 30 birds each. Anise seed powder was added at 0, 500, 750, and 1000mg/L to the drinking water offered to the C (control), T1, T2, and T3 groups respectively. The supplement was supplied for 56 days. When broilers were 28 and 56 days old, blood samples were collected (30 birds per group) by brachial vein puncture to evaluate the cellular components of blood (RBC, WBC, Hgb, HCT, H/L). The following serum biochemical parameters were evaluated: CHOL, GLU, TP, albumin, globulins, Ca, P, TG, total lipids, UA, and creatinine, as well as AST and ALT enzyme activities. The statistical analysis indicated that the anise supplement significantly improved blood RBC, WBC, Hgb, HCT, TP, albumin, globulin, GLU, P, and Ca levels of broilers in groups T4, T3, and T2 compared with the control group on days 28 and 56, and on average. Also, T3, T2 and T1 presented lower H/L ratio and CHOL, TG, total lipids, creatinine, UA, AST and ALT serum levels compared with the control group. The highest inclusion levels of anise seed powder, i.e., 1000 and 750mg/L, had a stimulating effect on the physiological traits of the birds.

摘要
本研究旨在探究添加于饮水中的茴芹（Pimpinella anisum L.）籽粉对肉用仔鸡部分血液指标的影响。本试验共选取360只1日龄哈伯德经典（Hubbard Classic）肉雏鸡，随机分为4组，每组设3个重复，每个重复30只鸡。分别向对照组（C组）、T1组、T2组及T3组的饮水中添加0、500、750及1000mg/L的茴芹籽粉，试验期共计56天。
分别在肉用仔鸡28日龄和56日龄时，通过肱静脉穿刺采集血液样本（每组采集30只鸡），以检测血液细胞成分：红细胞计数（Red Blood Cell, RBC）、白细胞计数（White Blood Cell, WBC）、血红蛋白（Hemoglobin, Hgb）、红细胞压积（Hematocrit, HCT）及异嗜性粒细胞与淋巴细胞比值（Heterophil-to-Lymphocyte ratio, H/L）。同时检测以下血清生化指标：总胆固醇（Cholesterol, CHOL）、葡萄糖（Glucose, GLU）、总蛋白（Total Protein, TP）、白蛋白、球蛋白、钙（Calcium, Ca）、磷（Phosphorus, P）、甘油三酯（Triglyceride, TG）、总脂、尿酸（Uric Acid, UA）、肌酐，以及天冬氨酸氨基转移酶（Aspartate Aminotransferase, AST）和丙氨酸氨基转移酶（Alanine Aminotransferase, ALT）的酶活性。
统计学分析结果显示，与对照组相比，T4、T3、T2组肉用仔鸡在28日龄、56日龄及整体平均水平下的血液RBC、WBC、Hgb、HCT、TP、白蛋白、球蛋白、GLU、P及Ca水平均显著提升。此外，T3、T2及T1组的血清H/L比值，以及CHOL、TG、总脂、肌酐、UA、AST及ALT水平均显著低于对照组。茴芹籽粉的最高添加水平（即1000mg/L和750mg/L）对肉用仔鸡的生理特性具有促进作用。