Transcriptomic profiling of CDCP1 knock-down in human cardiac fibroblasts (HCFs). Transcriptomic profiling of CDCP1 knock-down in human cardiac fibroblasts (HCFs)

To investigate the function of CDCP1 in regulation of HCF proliferation, we cultured primary HCFs and transfected with non-targeting siRNA and CDCP1 siRNAs. Total RNA samples were extracted for RNA-seq to identify transcriptome-wide gene expression changes in the HCFs after CDCP1 knock-down (KD). Overall design: Comparative gene expression profiling analysis of RNA-seq data for HCF cells with and wihout CDCP1 KD.

为探究CDCP1在调控HCF增殖中的功能，我们培养了原代HCF，并分别转染非靶向小干扰RNA（small interfering RNA, siRNA）与CDCP1靶向siRNA。随后我们提取总RNA样本进行RNA测序（RNA-seq），以鉴定CDCP1敲低（Knock-down, KD）后HCF中全转录组水平的基因表达变化。实验整体设计：对存在CDCP1敲低与未发生CDCP1敲低的HCF细胞的RNA测序数据开展比较基因表达谱分析。