Analysis of chromatin accessibility in human umbilical artery endothelial cells stimulated with BMP9 and fluid shear stress. Analysis of chromatin accessibility in human umbilical artery endothelial cells stimulated with BMP9 and fluid shear stress

Bone morphogenetic protein (BMP) signaling and fluid shear stress (FSS), the frictional force exerted on endothelial cells by blood flowing over them, have complementary functions in vascular homeostasis and disease development. Both induce a wide range of target genes, not only independently but also in a synergistic or antagonistic manner. Despite thorough research into genetic regulation downstream of BMP9 and FSS, detailed information on how both regulate chromatin accessibility is still missing. Here, we investigated whether BMP9 and FSS act independently, synergistically, or antagonistically in chromatin remodeling. To this end, we employed Assay for Transposase-Accessible Chromatin followed by sequencing (ATAC-seq) to analyze arterial endothelial cells stimulated with BMP9 and FSS either individually or in combination. Overall design: Bulk ATAC-seq of human umbilical artery endothelial cells (HUAECs) was performed in duplicates for a total number of 4 conditions (8 samples in total). Static, unstimulated cells were included as a control.

骨形态发生蛋白（Bone morphogenetic protein, BMP）信号通路与流体切应力（fluid shear stress, FSS）——即血液流经内皮细胞表面时对其施加的摩擦力——在血管稳态维持与疾病发生发展过程中具有互补调控功能。二者既可独立诱导大量靶基因表达，亦可通过协同或拮抗的方式共同调控靶基因的转录。尽管目前针对BMP9与FSS下游的遗传调控机制已开展了较为深入的研究，但关于二者共同调控染色质可及性的详细机制仍尚未明确。本研究旨在探究BMP9与FSS在染色质重塑过程中是否以独立、协同或拮抗的方式发挥作用。为此，我们采用转座酶可及性染色质测序测定（Assay for Transposase-Accessible Chromatin followed by sequencing, ATAC-seq）技术，对单独或联合经BMP9与FSS处理的动脉内皮细胞进行测序分析。实验整体设计：本研究对人脐动脉内皮细胞（human umbilical artery endothelial cells, HUAECs）进行批量ATAC-seq检测，共设置4种处理条件，每种条件设置2个生物学重复，总计8个样本；其中以静态未受刺激的细胞作为空白对照。