Results from the main gene expression analysis.

The Delta-Delta CT (ΔΔCT) relative quantification method was used to estimate mRNA levels of target genes relative to a reference gene (IPO8) in the small intestinal biopsies from patients with celiac disease and compared with controls. A total of 144 cases and 154 control samples were analyzed. The p-value is calculated using the independent samples t-test for equality of means (equal variances assumed).

本研究采用ΔΔCT相对定量法（Delta-Delta CT），以管家基因IPO8作为内参，对乳糜泻患者小肠活检组织中的靶基因mRNA相对表达水平进行估算，并与健康对照样本进行对比。本次分析共纳入144例患者样本与154例对照样本，显著性P值采用假设方差齐性的独立样本t检验进行计算。